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KMID : 1200820150150010083
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Oriental Pharmacy and Experimental Medicine
2015 Volume.15 No. 1 p.83 ~ p.93
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Protective effect Courouptia guianensis flower extract against N-nitrosodiethylamine-induced hepatic damage in wistar albino rats
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Rajesh Venugopalan
Kavitha Kuttappan Nair Valsala Kumari
Vishali Korrapati
Raju Chilukamari
Gayathri Kathirvel
Sruthi Akella
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Abstract
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The objective of the present study was to assess the hepatoprotective potential of Courouptia guianensis flower extract against N-nitrosodiethylamine (NDEA) induced hepatocellular damage. Hepatotoxicity was induced in albino rats by intraperitoneal injection of single dose of N-nitrosodiethylamine 200 mg/kg. Intraperitoneal injection of N-nitrosodiethylamine significantly elevated the levels of liver injury markers and liver cancer markers such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), Total bilirubin (TB), α-feto protein (AFP) and carcinoembryonic antigen (CEA) respectively. N-nitrosodiethylamine induced oxidative stress was confirmed by elevated levels of lipid peroxidation and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in liver tissue. The status of non-enzymatic antioxidants like, vitamin-C and vitamin- E were also found to be decreased in N-nitrosodiethylamine admininistered rats. However, post-treatment with Courouptia guianensis flower extract 200 mg/kg and 400 mg/kg orally for 30 days exhibited a significant protective effect by lowering the liver injury and liver cancer markers. On other hand, the extract treatment decreased the extent of lipid peroxidation and was able to normalize the activities of enzymatic and non-enzymatic antioxidants in liver. Gross examination and histological observations of liver tissue too correlated with the biochemical observations. These findings suggest that Courouptia guianensis flower extract possess hepatoprotective property against N-nitrosodiethylamine-induced liver damage which might be due to stabilization and increase in all the components of antioxidant system attributed to antioxidant and free radical scavenging activity.
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KEYWORD
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Courouptia guianensis, N-nitrosodiethylamine, Hepatic damage, Oxidative stress, Oxidant-antioxidant status
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