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KMID : 1200820150150020141
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Oriental Pharmacy and Experimental Medicine
2015 Volume.15 No. 2 p.141 ~ p.146
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Chemical composition and antimicrobial activity of Hyptis suaveolens Poit. seed oil from Uttarakhand State, India
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Bachheti R. K.
Rai Indra
Joshi Archana
Satyan R. S.
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Abstract
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The present study was carried out to identify the chemical composition and antimicrobial activity of Hyptis suaveolens Poit. seed oil from Kalagarh region (Uttarakhand State, India). Gas Chromatograph Mass Spectrum (GC-MS) analysis of the seed oil suggested high amounts (86.96 %) of unsaturated fatty acids: linoleic acid (76.13 %), oleic acid (10.83 %) compared to saturated fatty acids i.e. palmitic acid (6.55 %), stearic acid (4.56 %) and heptacosanoic acid (1.94 %) as the main constituents. The oil (1 mg/ml to 0.125 mg/ml) was detrimental to the growth of bacterial and fungal strains tested. Among them Salmonella typhi MTCC 531 (9.92?±?0.08 mm), Pseudomonas aeruginosa MTCC 424 (10.22?±?0.11 mm), Lactobacillus plantarum MTCC 2621 (10.92?±?0.09 mm) and Candida tropicalis MTCC 229 (8.11?±?0.07 mm) were susceptible at lower concentration (0.125 %). Escherichia coli MTCC 443, Shigella flexnerii MTCC 1457 and Vibrio vulnificus MTCC 1145 were sensitive only at higher concentrations (0.5 and 1 %) tested. The bioactivity of 1 mg/ml seed oil against C. tropicalis MTCC 229 was comparable to Fluconazole control (25 μg). On the contrary Enterococcus fecalis MTCC 439 and Streptococcus fecalis MTCC 3378 were resistant to the seed oil treatment. Minimum Inhibition Concentrations (MIC) were found to be 0.125 mg/ml for S. typhi, and P. aeruginosa, L. plantarum and C. tropicalis, while it was 0.2 mg/ml for L. leishmanii, S. aureus and C. albicans. The observations indicate that the H. suaveolens seed oil possess appreciable antimicrobial properties against Gram negative bacteria tested and can be further studied for the mode of action and further lead as a natural antibiotic.
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KEYWORD
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Hyptis suavolens Poit. seed oil, GC-MS, S. typhi, P. aeruginosa, C. tropicalis, MIC
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