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KMID : 1200820150150040305
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Oriental Pharmacy and Experimental Medicine
2015 Volume.15 No. 4 p.305 ~ p.312
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Fructose diet and low dose streptozotocin treatment induces the development of diabetic nephropathy in rats
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Patel Roshni
Shah Palak
Deshpande Shrikalp
Shah Gaurang
Gohil Priyanshee
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Abstract
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The aim of the present study is to find out the effect of fructose diet and low dose streptozotocin (STZ)-administration on development and progression of diabetic nephropathy (DN) in rats. Male Wistar rats were divided into four groups. Group I animals were fed with control diet and Group II, III, IV animals were fed with fructose diet throughout the study period. On 15th day of dietary manipulation, freshly prepared STZ (20 mg kg?1 and 30 mg kg?1, i.p.) was administered to Group III and IV animals, respectively. The fasting serum glucose, insulin, uric acid, urea, creatinine and urine albumin level was measured every 15 days till the end of experimental period. Histopathological evaluation of kidney tissues from each group was carried out on day 30, 45 and 60. Group II, III and IV showed hyperglycemia, hyperinsulinemia and deterioration of renal function markers compared with group I. Group IV demonstrated prominent characteristics of diabetic nephropathy and metformin (150 mg kg?1) treatment was initiated in this group from day 61 to 75. Metformin treatment significantly reduced FSG, insulin, uric acid and urea level. It was concluded that fructose fed-rats, injected with STZ (30 mg kg?1, i.p.) successfully induces diabetic nephropathy and mimic characteristics of DN in humans.
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KEYWORD
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Diabetic nephropathy, Fructose, Streptozotocin, Metformin, Rats
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