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KMID : 1200820150150040327
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Oriental Pharmacy and Experimental Medicine
2015 Volume.15 No. 4 p.327 ~ p.339
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Propensity of Hyoscyamus niger seeds methanolic extract to allay stereotaxically rotenone-induced Parkinson’s disease symptoms in rats
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Khatri Dharmendra Kumar
Juvekar Archana Ramesh
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Abstract
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Hyoscyamus niger (L), of Solanaceae family, commonly known as henbane is used in the traditional Indian medical system of Ayurveda and Chinese system of medicine for the nervous system disorders. We have evaluated neuroprotective potential of methanol extract of Hyoscymus Niger (MHN) seeds in stereotaxically induced rotenone model of Parkinson’s disease in rats. MHN was characterized employing HPLC-UV and LCMS. The extract showed presence of L-dopa with significant inhibition in DPPH, ABTS in-vitro assay and monoamine oxidase activity. Male Wistar rats were pretreated with MHN (125, 250, 500 mg/kg body weight p.o.) once daily for 7 days and subjected to unilateral intrastriatal injection of rotenone (8 μg in 0.1 % ascorbic acid in normal saline). Three weeks after rotenone infusion, rats were tested for neurobehavioral activity and were sacrificed for estimation of lipid peroxidation (TBARS), total glutathione (GSH) content, and activity of antioxidant enzymes glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) in brain homogenates. Administration of the MHN (containing L-DOPA) significantly attenuated motor disabilities (actophotometer, rotarod and Morris water maze test). Rat treated with rotenone showed reduced levels of thiobarbituric acid reactive substance (TBARS) and increased level of GSH content and antioxidants enzymes activities (GPX, SOD and CAT) in the MHN treated PD rat. The findings suggest that MHN is a potential drug for treating oxidative damage, physiological abnormalities and is effective in neuroprotection in experimental models of PD.
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KEYWORD
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Rotenone, Parkinson’s disease, Hyoscyamus Niger, MAO-B, Antioxidant
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