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KMID : 1200820160160030195
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Oriental Pharmacy and Experimental Medicine
2016 Volume.16 No. 3 p.195 ~ p.201
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Antimicrobial activity of green silver nanoparticles produced using aqueous leaf extract of Hydrocotyle rotundifolia
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Kumari Ragini
Brahma Girija
Rajak Sumit
Singh Mukesh
Kumar Santosh
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Abstract
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In the present work, colloidal silver nanoparticles (AgNPs) were synthesized in a single step process by green biosynthetic method using aqueous leaf extracts of an abundantly available medicinal plant Hydrocotyle rotundifolia. The AgNPs synthesized were characterized by UV-Vis spectroscopy, Transmission Electron Microscopy (TEM), Energy-Dispersive X-ray (EDX), Selected Area Electron Diffraction (SAED) and Fourier transform infrared (FTIR) spectroscopy. The UV-Vis absorption spectra of colloidal silver nanoparticles showed characteristic surface plasmon resonance (SPR) peak centred at 410 nm. The high resolution TEM images showed the as-synthesized nanoparticles are mostly spherical with and some elliptical in shape having average diameter of about 7.39 nm. FTIR analysis clearly revealed that the biomolecules present in the leaf extract of H. rotundifolia could be responsible for reduction of silver ion as well as prolonged stability of nanoparticles. The as-synthesized nanocolloids were stable and no precipitation was observed up to 3-months incubation at room temperature. The synthesized AgNPs were tested for its antimicrobial property against E. coli (DH5α). The MIC value was recorded as 5 μg/mL and demonstrated significant growth inhibition on agar plate. This eco-friendly and economical method may be a good option for the large scale preparation of silver nanoparticles for application in pharmaceutical, food, textiles and cosmetic industries.
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KEYWORD
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Biosynthesis, Hydrocotyle rotundifolia, Medicinal plant, Silver nanoparticle, FTIR analysis
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