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KMID : 1200820160160040333
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Oriental Pharmacy and Experimental Medicine
2016 Volume.16 No. 4 p.333 ~ p.337
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Antiplatelet activity and isolation of triterpenoidal glycoside from ethanolic extract of Nepeta hindostana
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Ansari Vaseem A.
Jawaid Talha
Siddiqui H. H.
Dixit R. K.
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Abstract
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In the present study, triterpenoidal glycoside was isolated from ethanolic extract of aerial part of the plant Nepeta hindostana. The ethanolic extract was subjected to Si-gel CC and compound was isolated. The isolated compound Napedinolside has been characterized as 1β,11α,30-trihydroxy-lup-20(29)ene-(3?→?1′)-β-D-pyranoside on the basis of spectroscopic (UV, IR, 1H, 13C NMR and MS) methods. The ethanolic and aqueous extract at different concentrations were screened for antiplatelet activity using platelet rich plasma (PRP) prepared by centrifugation of rabbit whole blood (containing 0.9 % sodium citrate as anticoagulant) at 1500 rpm for 15 mins. The Np-01 was identified as triterpenoidal glycoside which was amorphous brownish white powder; yield 0.64 % (w/w); mp 125?127. N. hindostana showed very excellent antiplatelet activity in both aqueous and alcoholic extracts. The minimum concentration showed antiplatelet activity was 0.5 μg/ml. These findings demonstrate that ethanolic and aqueous extract of the selected plant material at different concentrations showed significant antiplatelet activity. The triterpenoidal glycoside from aerial parts of N. hindostana could hold promise for future application in the treatment of blood clot and pulmonary embolism related diseases.
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KEYWORD
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Nepeta hindostana, Triterpenoidal glycoside, Antiplatelet activity, Platelet rich plasma
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