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KMID : 1200820170170010029
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Oriental Pharmacy and Experimental Medicine
2017 Volume.17 No. 1 p.29 ~ p.39
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Alpha-glucosidase inhibition and antihyperglycemic activity of flavonoids rich fractions of Rosmarinus officinalis in normal and streptozotocin diabetic mice
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Belmouhoub Messaoud
Bribi Noureddine
Iguer Ouada Mokrane
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Abstract
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The aim of the present study was to evaluate the antihyperglycemic effect of flavonoids rich fractions of Rosmarinus officinalis L. namely n-butanol fraction (BUT) and diethyl ether fraction (D.E). Antihyperglycemic action was studied in vitro through the inhibitory tests on α-glucosidase of Saccharomyces cerevisiae. In vivo, the antidiabetic effects were tested in both normal and streptozotocin (STZ) induced diabetic mice using various tests including: the decrease of post prandial hyperglycemia, oral glucose tolerance test (OGTT), hypoglycemic effect and inhibition of glucose intestinal transporters. The in vitro α-glucosidase inhibition activity showed that both fractions (BUT and D.E) presented a potent effect. The maximum inhibition with D.E and BUT fractions was, 77 and 72% at 250 μg/ml, respectively. In vivo, BUT fraction at 800 mg/kg, showed a significant impact on post prandial hyperglycemia with 40.77% and 28.2% as a maximum glucose level reduction using sucrose and maltose, respectively. In OGTT, the maximum antihyperglycemic effect (51.65%) was also obtained with BUT fraction at 800 mg/kg. When testing the impact on glucose intestinal transport, a dose of 800 mg/kg of D.E fraction exhibited a significant inhibition with 24.12% as a maximum reduction. The present results clearly demonstrated the complementary effect of Rosmarinus officinalis flavonoids rich fractions, resulting to α-glucosidase inhibition and to antihyperglycemic action through glucose transport inhibition and blood glucose level reduction.
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KEYWORD
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Rosmarinus officinalis, OGTT, Intestinal glucose transport, Streptozotocin, Diabetic mice, Postprandial hyperglycemia
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