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KMID : 1200820170170010071
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Oriental Pharmacy and Experimental Medicine
2017 Volume.17 No. 1 p.71 ~ p.80
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Solanum trilobatum beneficially modulates dimethyl benz (a)anthracene induced skin carcinogenesis and erhlich Ascites cell induced solid tumor in mice
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Sini H.
Devi K. S.
Nevin K. G.
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Abstract
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The aim of this study was to assess the potential of chloroform extract of S. trilobatum (CST) on 7,12-dimethyl benz (a)anthracene (DMBA) induced skin carcinogenesis and Ehrlich ascites carcinoma cells (EAC) induced solid tumor in mice. CST was obtained by exhaustive extraction with chloroform. Skin carcinogenesis was induced in Swiss albino mice by applying DMBA, while solid tumor was induced by intramuscular injection of EAC cells. For preventing skin carcinogenesis, animals were treated with CST (5 mg/kg body weight/100 μl acetone) before and after DMBA application for 15 days. Solid tumor was treated with CST (400 mg/kg body weight) prophylatically and post inoculation. After the experimental period, several anti tumor parameters and antioxidant status of skin of treated and control animals were determined. Solid tumor progression was determined by measuring the tumor diameter for 36 days. CST was sub-fractionated with different solvents and each fraction was subjected to HPLC-MS. HPLC-MS analysis showed the presence of two components with possible anti-cancer activity viz. 3,5,7 trimethoxy flavone and vitexin per-O-acetyl. The biochemical results showed that CST treatment reduced the number of skin papillomas, tumor incidence as well as modulated the antioxidant status of the skin in a beneficial way. CST reduced the solid tumor volume induced by EAC significantly. In conclusion, CST with its biologically active components may be used as a part of cancer treatment.
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KEYWORD
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Solanum trilobatum, Dimethylbenz(a)anthracene, Skin cancer, Solid tumor, Phytochemicals
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