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KMID : 1200820170170030233
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Oriental Pharmacy and Experimental Medicine
2017 Volume.17 No. 3 p.233 ~ p.238
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In vitro cytotoxic and antioxidant activities of Pikut Trichinthalamaga remedy
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Dechayont Bhanuz
Limpichai Chayaporn
Kornwisitwathin Kornrawee
Nuengchamnong Nitra
Itharat Arunporn
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Abstract
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The Pikut Trichinthalamaga remedy appears in ancient Thai pharmacy scripture, as a purgative, and includes Rhubarb (Rheum palmatum L.), Myrabulan fruit (Terminalia chebula Retz.), and Gamboge (Garcinia handuryi Hook F.). We investigated the cytotoxic properties of the herbal extracts using the SRB assay in human colon adenocarcinoma cell lines (LS174T and SW480) and normal lung fibroblast cell (MRC-5) as well as their antioxidant activities by chemical-(ABTS assay) and cell-based assays [nitric oxide (NO) assay]. The active fractions were identified using high-performance liquid chromatography coupled to electrospray ionisation and quadruple time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). The ethanolic extract of Pikut Trichinthalamaga remedy had a cytotoxic effect on LS174T and SW 480 cells (IC50 = 0.39 and 0.51 μg/ml, respectively). The ABTS assay showed that the water extract of the myrabulan fruit had the highest activity (EC50 = 5.69 μg/ml) while the NO assay showed that the ethanolic extract of Pikut Trichinthalamaga remedy had the most potent inhibitory activity on NO production (EC50 = 0.62 μg/ml). After liquid?liquid partitioning sequence, the chloroform fraction had the highest cytotoxicity. Emodin, forbesione, gaudichaudionic acid and gaudichaudionol were identified in this extract. These results support the further investigation of the Pikut Trichinthalamaga remedy as a potential anticancer drug.
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KEYWORD
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Cytotoxicity, Antioxidant activity, Pikut Trichinthalamaga remedy, Rheum palmatum, Terminalia chebula, Garcinia hanburyi
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