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KMID : 1200820180180010059
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Oriental Pharmacy and Experimental Medicine
2018 Volume.18 No. 1 p.59 ~ p.66
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In vitro antibacterial activity of flavonoids extracts from three Algerian horehound (Marrubium vulgare L.) leaves
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Bouterfas Karim
Mehdadi Zoheir
Elaoufi Manal Maliha
Aouad Linda
Latreche Ali
Benchiha Walid
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Abstract
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Marrubium vulgare L. is used in folk medicine of several countries. Many researches showed that it is used for treatment of variety of diseases, for example as remedy for asthma and diabetes, duo to its antibacterial effects. The present study aimed to access the effect of the sampling location on the antibacterial activity of the flavonoids extracted from Marrubium vulgare L. leaves, against three pathogenic bacteria; Escherichia coli, Bacillus cereus, and Proteus mirabilis. The leaves were collected from three different sampling locations belonging northwest Algeria: Tessala Mountain, M’sila forest and Ain Skhouna. The flavonoid extraction was carried out using organic solvents with increasing polarity. The bacterial susceptibility testing by diffusion agar method showed that the diameters zones varied significantly (P < 0.001) according to the sampling location of the leaves, the flavonoid extract and its concentration. The inhibition diameters varied between 7.5?34.3 mm, which often exceed those induced by the standard antibacterial agents (chloramphenicol, gentamicin, aztreonam, nalidixic acid, ceftazidime, and imipenem). The agar dilution method showed that the minimum inhibitory concentrations (MICs) range between 25 and 100 μg/mL; revealing strong antibacterial inhibition. The phytochemical screening revealed the presence of flavans and flavanols, which may be responsible of this promising antimicrobial activity.
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KEYWORD
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Marrubium vulgare, Flavonoid extracts, Antibacterial activity, Sampling location
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