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KMID : 1200820200200010115
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Oriental Pharmacy and Experimental Medicine
2020 Volume.20 No. 1 p.115 ~ p.122
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Antimicrobial, cytotoxic, phytochemical and biological properties of crude extract and solid phase fractions of Monotheca buxifolia
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Ali Joham Sarfraz
Khan Ihsan
Zia Muhammad
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Abstract
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The present study revolves around two different parts of Monotheca buxifolia extract and their solid phase extraction; and phytochemical and biological evaluation. Polar solvents tended to give maximum extraction yield. The highest phenolic content (69.1?±?1 μgGAE/mg extract) was observed in the ethyl acetate?+?methanol (EtOAc?+?MeOH) fraction of stem. While highest flavonoid content (52.1?±?0.45 μg QE/mg extract) was observed in MeOH crude extract of leaf. The antioxidant capacity was depicted by the MeOH fraction of leaf and EtOAc + MeOH fraction of stem. MeOH crude extract of leaf and CHCl3 (chloroform) crude extract of stem had notable inhibitory effect against bacterial strains (MIC 11.1 and 23.2 μg/ml, respectively). CHCl3 extract of leaf and n-hexane fraction presented significant antifungal activities (MIC 11.1 ug/ml).Though protein kinase inhibition was not significant but significant antileismanial activity and cytotoxic activity was observed. The study demonstrates potential of M. buxifolia for discovery of significant bioactive compounds through bioactivity guided isolation. Furthermore solid phase extraction is better for partial fractionation of active component.
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KEYWORD
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Phytochemical, Antioxidant, Antileishmanial, Antimicrobial
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