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KMID : 1200820200200040619
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Oriental Pharmacy and Experimental Medicine
2020 Volume.20 No. 4 p.619 ~ p.630
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Anti-apoptotic and anti-inflammatory activity of Gentiana lutea root extract
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Cafaro Teresa
Carnicelli Veronica
Caprioli Giovanni
Maggi Filippo
Celenza Giuseppe
Perilli Mariagrazia
Bozzi Argante
Amicosante Gianfranco
Brisdelli Fabrizia
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Abstract
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Gentiana lutea roots have been widely used in the traditional medicine, especially for their stomachic properties. In this study, the effect of G. lutea root methanol extract on SH-SY5Y cell survival and on anti-proliferative activity of pro-apoptotic agents was evaluated. In neuroblastoma cell line SH-SY5Y, the extract did not exhibit any cytotoxic activity, but protected cells from vinblastine-induced apoptosis. In the combined treatment with 200 μg/ml extract plus 0.1 μM vinblastine, extract significantly increased cell survival, reduced the percentage of apoptotic cells and decreased caspase-3 activity if compared with the vinblastine alone treatment. The anti-apoptotic activity of the extract was associated to the down-regulation of Sirt-1 protein and to the increase of anti-apoptotic Bcl-2 protein expression and intracellular GSH levels. Since the apoptosis modulation was evidenced on a neuroblastoma cell line, G. lutea extract could have a promising potential as a neuroprotective agent. Moreover, G. lutea root extract exhibited anti-inflammatory activity, since it inhibited LPS-induced expression of TNF-α in macrophage RAW264.7 cells.
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KEYWORD
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SH-SY5Y cells, Apoptosis, Vinblastine, Gentiana lutea, Anti-inflammatory activity
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