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KMID : 1200820210210030475
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Oriental Pharmacy and Experimental Medicine
2021 Volume.21 No. 3 p.475 ~ p.484
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Stability of cannabidiol, ?9-tetrahydrocannabinol, and cannabinol under stress conditions
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Yangsud Jiroj
Santasanasuwan Supadit
Ahkkarachinoreh Pongphop
Maha Athip
Madaka Fameera
Suksaeree Jirapornchai
Songsak Thanapat
Vutthipong Arthimond
Monton Chaowalit
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Abstract
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The aim of this work was to validate a high-performance liquid chromatography (HPLC) method for determination of the stability of cannabidiol, ?9-tetrahydrocannabinol, and cannabinol. Furthermore, degradation kinetics were also investigated. Five stress conditions?acid degradation, alkaline degradation, oxidation, thermal degradation, and photodegradation?were evaluated. The results showed that the HPLC method had a linear response (R2?≥?0.9999) in the test range of 1?200 μg/mL. The method was specific, precise, and accurate. The limits of both detection and quantitation are also reported. According to the stress test, the three cannabinoids (cannabidiol, ?9-tetrahydrocannabinol, cannabinol) were stable during exposure to a range of thermal conditions for 24 h. They were unstable when being subjected to acid conditions; cannabidiol under alkaline conditions was extremely unstable. Degradation kinetic analysis demonstrated that the compounds remained at a level of approximately 8% after 5 h, and approached a first-order reaction (R2?=?0.9930) with a rate constant of ? 0.5057 h??1. In summary, the obtained data can be used as a guide for the formulation development of cannabis products in order to maintain their active compounds as well as their activities.
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KEYWORD
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Cannabinoids, Cannabis, Method validation, Stability, Stress test
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