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KMID : 1200820210210040791
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Oriental Pharmacy and Experimental Medicine
2021 Volume.21 No. 4 p.791 ~ p.803
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Morphological and cytogenetic analysis of different cytotypes of Boerhaavia diffusa L. and their evaluation for biological activity
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Sharma Nisha
Singh Bikram
Bhatia Aruna
Gupta Raghbir Chand
Wani Mohammad Saleem
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Abstract
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Boerhavia diffusa is an herbaceous plant of the family Nyctaginaceae. It is mentioned to have anti-aging, disease prevention, and life-enhancing activities that hold enormous influence on disease burden and affordability/availability of healthcare in the world. In the present study, various populations of this plant species were characterized using morphological markers, cytogenetic and phytochemical analyses. Besides, biological activity was also studied for anti-diabetic and anti-bacterial effects using rats and bacterial systems respectively. For the three cytotypes i.e. diploid, tetraploid and octaploid, twelve morphological traits were studied. HPLC system was used for the identification of bioactive compounds present in extracts. The in vivo anti-hyperglycemic effects of the extract in alloxan- induced diabetes were determined using Swiss albino rats. Rats with fasting plasma glucose vary?≥?300?315 mg/dl were treated to be diabetic and utilized for the examination. Anti-bacterial activities of three cytotypes of B. diffusa were screened by using the Disc diffusion method against two strains of Gram-positive bacteria and three strains of Gram-negative bacteria. The present study reports the existence of diploid, tetraploid and octaploid cytotypes which was new report from India. The diploid cytotype of B. diffusa showed maximum activity against diabetes in alloxan-induced diabetic rats and more anti-bacterial activity in Gram-positive and Gram-negative bacteria as compared to tetraploid and octaploid cytotypes. HPLC results showed that the concentration of phytoconstituents showed a negative correlation with the ploidy level being maximum in plants with lower ploidy levels. The present paper represents the first report of the pharmacognostic study based on different plant parts and cytotypes in B. diffusa. Results of the present study will be useful for exploiting appropriate cytotypes for various purposes such as formulating new anti-diabetic and anti-bacterial drugs from B. diffusa.
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KEYWORD
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Boerhaavia diffusa, Morphology, Cytology, HPLC, Biological activity
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