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KMID : 1200820220220020371
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Oriental Pharmacy and Experimental Medicine
2022 Volume.22 No. 2 p.371 ~ p.382
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Optimization of plant compositions of Trisattakula to maximize antibacterial activity and formulation development of film-forming polymeric solution containing Nigella sativa ethanolic extract
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Monton Chaowalit
Settharaksa Sukanya
Suksaeree Jirapornchai
Chankana Natawat
Charoenchai Laksana
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Abstract
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The Trisattakula herbal recipe has been used for a long time for the treatment of the symptom of the gastrointestinal tract. Based on the plant compositions, it exhibits antibacterial activity. This work sought to optimize the mass ratios of plant compositions of Trisattakula recipe (equal mass ratios of dried seeds of Nigella sativa L., dried fruits of Coriandrum sativum L., and fresh rhizomes of Zingiber officinale Roscoe) to maximize the anti-Staphylococcus aureus and anti-S. epidermidis activities. The simplex lattice design was applied in the optimization process. Results showed that dried seeds of N. sativa alone gave the highest extraction yield, and the extract of dried seeds of N. sativa alone exhibited the most potent antibacterial activity. The ethanolic extract of dried seeds of N. sativa was further used as an active ingredient of film-forming polymeric solution (FFPS). The three film-forming agents, including hydroxypropyl methylcellulose E5, xanthan gum, and Eudragit® RL 30D, were used. However, 5% hydroxypropyl methylcellulose E5 blended 0.5% xanthan gum FFPS system containing 9.2% N. sativa extract was the best formulation. It showed good antibacterial activity against both S. aureus and S. epidermidis. The release of thymoquinone determined using validated high-performance liquid chromatography approached the Korsmeyer-Peppas model. In summary, the FFPS containing N. sativa extract could be used as an alternative antibacterial product for the treatment of skin infection caused by the two pathogens: S. aureus and S. epidermidis.
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KEYWORD
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Trisattakula, Film-forming polymeric solution, Nigella sativa, Optimization, Antibacterial activity, Thymoquinone
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