|
KMID : 1200820220220030581
|
|
Oriental Pharmacy and Experimental Medicine
2022 Volume.22 No. 3 p.581 ~ p.588
|
|
|
8C-glycosylflavone from the Piper auritum suppressed glycoxidation process in vitro and in streptozotocin-induced diabetic mice
|
|
Gutierrez Rosa Martha Perez
|
|
|
Abstract
|
|
|
|
Investigated the effects with C-glycosylflavone against the advanced glycation end-product (AGE) in vitro and in vivo. Chromatographic fractionation of the MeOH extract of P. auritum led to the isolation of the known 5,3′,4′-trihydroxy-7,8-dimethoxy-6-C-[β-D- xsylopyranosyl -(1?→?2)]-β-D-glucopyranosyl flavonoside (1). The structure was established on the basis of spectroscopic methods. Was investigate the effects of 1 against the advanced glycation end-product (AGE) in vitro and in vivo in a mice model of streptozotocin (STZ)-induced diabetes. Flavonoid significantly reduced the formation of fluorescent (AGEs, AOPPs), no fluorescent (Nε-CML), and fructosamine level in AGEs-BSA system. Its also avoided oxidation protein products, which was shown by reduction of protein carbonyl, and increase of thiol group, decreased the level of amyloid cross β-structure and suppressed the protein carbonylation in MGO-BSA model. Oral administration of 1 produced a significant decrease of serum glucose, inhibit the formation of GlyHb, prevented circulatory AGEs level, attenuated accumulation of AGEs in liver and kidney and reduced lipids accumulation possibly due to a better functioning of β-cell in diabetic mice. Finding, indicated that 1 can alleviating these parameters through diverse mechanisms with a marked antiglycation activity. Flavonoid participate in the initial and intermediate stages of glycation, leading to reduction of the production of AGEs in the late stage. Results indicated that 1 improving AGEs formation in type 2 diabetes.
|
|
|
KEYWORD
|
|
|
Piper auritum, Glycation, Diabetes, Glycoxidation, Flavonoids
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|