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KMID : 1201620100030020057
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Journal of Women s Medicine
2010 Volume.3 No. 2 p.57 ~ p.62
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Comparison of human sperm quality and nuclear DNA integrity between slow and rapid freezing
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Jee Byung-Chul
Chang Hye-Jin
Jeon Yong-Tark
Lee Jung-Ryeol
Suh Chang-Suk
Kim Seok-Hyun
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Abstract
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Objective: To compare human sperm quality and nuclear DNA integrity after cryopreservation either by slow or rapid freezing.
Methods: Fifty?four normozoospermic males were recruited in this prospective comparative study. Sperm samples were divided into two aliquots and then cryopreserved either by slow (using programmable freezer) or rapid freezing (direct plunging cryotubes into liquid nitrogen). Sperm quality was assessed by World Health Organization criteria and nuclear DNA integrity was measured by the terminal deoxynucleotidyl transferase (TdT)?mediated deoxyuridine triphosphate (dUTP) nick?end labeling (TUNEL) assay before and after cryopreservation.
Results: In slow freezing group, sperm motility was significantly higher (37.4±14.7% vs. 34.6±14.3%, P<0.05) and sperm nuclear DNA fragmentation was significantly lower (15.5±9.8% vs. 16.8±10.3%, P<0.05) when compared with rapid freezing group. Especially in asthenozoospermic samples, motility?conserving effect was striking by slow freezing method.
Conclusion: Slow freezing method is preferred for cryopreservation of human sperm in normozoospermic men, especially with low motility.
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KEYWORD
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Sperm, Slow freezing, Rapid freezing, Nuclear integrity, DNA fragmentation
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