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KMID : 1204320060220040339
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Laboratory Animal Research
2006 Volume.22 No. 4 p.339 ~ p.342
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Local Irritation Test of 3 Types of β-Glucan after Subcutaneous Injection in Rats
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Lee Hyeung-Sik
Ku Sae-Kwang
Yang Kun-Ju
Shin Hyun-Dong
Park Bok-Ryeon
Cho Kwang-Ho
Kim Hyeong-Dong
Cho Hyung-Rae
Jang Hee-Jeong
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Abstract
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The objective of the present study is to detect the potency of skin irritation of 3 types of β-glucan: Polycan, Tinocare GL and SC-glucan, after a subcutaneous injection at 2 g/kg, respectively. Test articles were given single subcutaneous injection at the shaving back of rat one point per rat and they were sacrificed at 7 days after injection. Histopathological changes on the injection sites were observed with histomorphometry, the thickness of encapsulation and percentages of collagenous tissues in encapsulation regions. As results of subcutaneous injection of all three types of β-glucan, encapsulations consisted of inflammatory cells and connective tissues caused by skin irritation and foreign body reactions, were observed with microangiogenesis restricted to the injection sites. The most severe histopathological changes were detected in the SC-glucan and then in order of Tinocare GL and Polycan. Therefore, it is considered that the potency of the skin irritant caused by β-glucan is quite differed from their origin and they showed relatively lower irritation because the changes related to the irritation and foreign body reactions were restricted to the injection sites. Among three types of β-glucan tested in the present study, Polycan showed the lowest irritation and SC-glucan showed the highest skin irritation.
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KEYWORD
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Histopathology, skin irritation, Polycan, Tinocare GL, SC-glucan
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