|
KMID : 1204320060220040349
|
|
Laboratory Animal Research
2006 Volume.22 No. 4 p.349 ~ p.353
|
|
|
Helicobacter pylori Infection Induces Inflammatory Cytokine Production in Alternative Model to Animal Tests for Assessing the Pathogenesis
|
|
Suzuki Hidekazu
Kim Ok-Jin
|
|
|
Abstract
|
|
|
|
Based on the central role of cells in the expression of toxicity and pathogenesis, several mammalian in vitro models have received regulatory acceptance by the Organization for Economic Co-operation and Development (OECD) as alternatives to whole animal tests in human health science. In this study, we used in vitro cell culture system to study the effect of H. pylori infection in gastric cells. Two kinds of gastric cell lines (mucous and parietal cells) were infected with H. pylori organisms of previously defined cagE and cagA status. The production of inflammatory chemokine was measured in cultured cells using RTPCR and the amounts of expressed mRNAs were evaluated by semi-quantitative analysis using NIH image program. Both mucous and parietal cell lines produced inflammatory chemokine, especially macrophage inflammatory protein-2 (MIP-2), by H. pylori infection. However, the expressed amounts were higher in parietal cells than mucous cells. In this study, we establish alternatives to animal tests for assessing the pathogenesis by H. pylori infection. The mouse MIP-2 was considered functional homologues of human IL-8 in mice. It is concluded that inflammatory chemokine is expressed strongly by parietal cells following H. pylori infection. It may contribute to inflammation present in gastric mucosa associated with H. pylori infection. On our knowledge, this report is first report of the effect of gastric parietal cells by H. pylori infection.
|
|
|
KEYWORD
|
|
|
Cell culture, Helicobacter, parietal cell, inflammation, cytokine, in vitro
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|