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KMID : 1204320070230010069
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Laboratory Animal Research
2007 Volume.23 No. 1 p.69 ~ p.75
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Effects of Licorce on the Gene Expression of Glutathione Peroxidase Family in the Reproductive Systems of Male Rats
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Lee Se-Ra
Nam Sang-Yoon
Lee Beom-Jun
Kim Yun-Bae
Ahn Byeong-Woo
Baek In-Jeoung
Yon Jung-Min
Kim Mi-Ra
Jin Yan
Yun Young-Won
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Abstract
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Licorice, the root of the leguminous Glycyrrhiza, is one of the most frequently employed botanicals in foods and traditional medicines, which is composed of triterpene saponins, flavonoids, polysaccharides, pectins, simple sugars, amino acids, mineral salts, and various other substances. To investigate whether licorice affects on the antioxidant system of male reproductive organs, we analyzed the mRNA expression pattern of glutathione peroxidase (GPx) family (type 1-4) in the reproductive tissues including testes, epididymides, prostates, and seminal vesicles of rats exposed to licorice. Licorice extracts (0, 0.5, 1, and 2 g/㎏ body weight) were treated to 6-week-old-male rats for 4 weeks daily per oral. Total RNAs were extracted from the male reproductive tissues, and then RT-PCR was performed with the specific primers of each GPx enzyme. The GPx1 mRNA expression was similar to control group in testes, epididymides, and seminal vesicles, but was significantly increased in the prostates exposed to 0.5, 1, and 2 g/㎏ of licorice. There was no significant difference in the expression of GPx2 mRNA among control group and licorice-treated groups. The GPx3 mRNA was weakly increased except for 2 g/㎏ group in testes, but was weakly decreased in epididymides. GPx4 mRNA was not changed in testes, but was slightly decreased in epididymides, seminal vesicles, and prostates by 0.5 and 2 g/㎏ of licorice. These findings indicate that licorice affects the expression of GPx genes in male reproductive system.
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KEYWORD
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Licorice(glycyrrhiza glabra), glutathione peroxidase(GPx), male reproductive organs
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