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KMID : 1204320070230020117
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Laboratory Animal Research
2007 Volume.23 No. 2 p.117 ~ p.120
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Streptozotocin-Induced Diabetes Decreases Proliferating Cell Nuclear Antigen in Rat Testes
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Koh Phil-Ok
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Abstract
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Sexual dysfunction is associated with diabetes in males. The present study was designed to evaluate whether streptozotocin-induced diabetes decreases proliferative activity in rat testes. Diabetes was induced by a single intravenous injection of streptozotocin (40 ㎎/㎏ body weight). Testes samples were collected after 3 months. Testicular weight decreased in the diabetic group. Proliferating cell nuclear antigen (PCNA) was used as a proliferative marker. Western blot analysis showed that the level of PCNA significantly decreased in diabetic group, compared to that of controls. The level of PCNA was 1.12 ± 0.04 and 0.87 ± 0.11 in control and diabetic rats, respectively. Also, PCNA-positive cells in the spermatogonia and primary spermatocytes decreased in diabetic rats. The present study demonstrates that streptozotocin-induced diabetes decreases the cell proliferative activity in testes. In conclusion, our findings suggest that decrease of cell proliferation in the testes of diabetics contributes to the suppression of male reproductive activity.
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KEYWORD
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Diabetes, PCNA, testes
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