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KMID : 1204320070230020149
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Laboratory Animal Research
2007 Volume.23 No. 2 p.149 ~ p.154
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Immunohistochemical Study of Osteopontin in Rat Brain with C6 Gliomas
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Kim Hee-Chul
Sim Ki-Bum
Shin Tae-Kyun
Nam Do-Hyun
Kim Seung-Joon
Lee Yong-Duk
Kim Jeong-Tae
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Abstract
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Immunohistochemical analysis of osteopontin (OPN) was studied in the brain of rats with implanted C6 glioma to see the involvement of OPN in the pathogenesis of C6 glioma in in vivo. Three lesions including tumor cell infiltration area, compact tumor growth area and contralateral area were compared at day 11 post-implantation. OPN was immunostained in the extracellular matrix, as well as in some ED1 (anti-rat macrophage)-positive macrophages/activated microglia, but few in astrocytes, in the tumor cell infiltration area. In the compact tumor growth area, some glial fibrillary acidic protein-positive glioma cells were also stained for OPN, while OPN immunoreactivity was very weak in ED1 positive macrophages. In contralateral lesion of C6 glioma implantation and normal control brains, OPN was only found in few neurons and vascular endothelial cells, but not in glial cells. These results suggest that OPN may be involved in the tumor-associated activation of macrophages, particularly in the lesion of tumor infiltration, and be involved in the proliferation and expansion of astrocytes in the course of rat C6 glioma models.
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KEYWORD
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Astrocytes, C6 glioma, macrophages, osteopontin
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