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KMID : 1204320070230030231
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Laboratory Animal Research
2007 Volume.23 No. 3 p.231 ~ p.238
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Differential Interaction between Selenoprotein M and Apoptosis-Related Proteins in HEK 293 Cell and Transgenic Rat Overexpressing the Human Selenoprotein M
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Hwang Dae-Youn
Cho Jung-Sik
Jee Seung-Wan
Kim Yong-Kyu
Shim Sun-Bo
Lee Su-Hae
Sin Ji-Soon
Kim Chuel-Kyu
Kim Byung-Guk
Bae Chang-Jun
Lee Byoung-Chun
Jang Mee-Kyung
Kim Min-Sun
Yim Su-Youn
Chae Kab-Ryong
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Abstract
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Selenoprotein M (SeIM) is a eukaryotic selenoprotein containing one selenocysteine (Sec) and Sec insertion sequence (SECIS) in 3’ untranslated regions. In order to examine whether SelM expression and selenium treatment were influenced on expression level of the apoptosis-related proteins, pCMV/hlDE fusion gene were overexpressed in the HEK 293 cell line and hSelM-Tg rat. Herein, it is concluded that; ⅰ) two transgenic mice were identified by PCR analysis using specific primers by screening 28 newborn founder mice, ⅱ) the GFP-hSeIM fusion protein were successfully detected on the protein level in the HEK 293 cell line and hSeIM-Tg rat. ⅲ) it was also observed that p53 proteins were significantly changed in the HEK 293 cell under the condition of hSeIM overexpression and selenuim treatment, while not changed in the kidney tissue of hSeIM-Tg rat. ⅳ) Bax proteins were significantly changed in the kidney of hSeIM-Tg rat under the condition of hSeIM overexpression and sel treatment, while not changed in the HEK 293 cell line. ⅴ) Bel2 proteins in the HEK 293 cell were significantly decreased in the hSeIM transfectants, but hSeIM-Tg rat were shown the different expression patterns in the sel treated condition. These results suggest that selenium treatment and SeIM overexpression were differently influenced the expressions of apoptosis-related proteins in the tumor cell line and transgenic tissue
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KEYWORD
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Selenoprtien M, p53, Bax, Bcl2, transgenic rat
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