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KMID : 1204320070230030277
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Laboratory Animal Research
2007 Volume.23 No. 3 p.277 ~ p.283
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Effects of Major Histocompatibility Complex Difference on Systemic Anaphylaxis Occurrence in Mice
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Park Hyun-Jin
Hur Sook-Jin
Kim Hyoung-Ah
Heo Yong
Lee Sang-Koo
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Abstract
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This study was undertaken to investigate contribution of major histocompatibility complex difference on systemic anaphylaxis occurrence related with vaccination. Systemic anaphylaxis has been induced in BALB/c (lgh-1a,H-2d), DBA/2(lgh-1c,H-2d), C3H (H-2k), and C57B6 (H_2b) mice through intraperitoneal sensitization with chicken egg ovalbumin (OVA) followed by intravenous challenge with OVA. The magnitude of anaphylaxis occurrences was determined by measuring levels of plasma histamine, IgE, OVA-specific IgG1/G2a, total IgG1/G2a, cytokine production from splenocytes stimulated in vitro. In the experiment to compare the levels of histamine and IgE between the anaphylactic and adjuvant control mice, the efficacy of upregulation of histamine and IgE was in the order of DBA2 ＞ BALB/c ＞ C57BL/6 ＝ C3H/He mice. Furthermore, relative level of OVA-IgG1 over OVA-IgG2a was also the most prominent in the DBA/2 mice. Order of IL-4 versus IFN-? ratio, which indicates skewing of helper T cell reactivities, was DBA/2 ＞ BALB/c ＞ C57BL/6,C3H/He mice. This study endows the scientific evidence on a hypothesis that differences in the immuno-genetic background could significantly contribute the occurrence of systemic anaphylaxis observed following various vaccinations.
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KEYWORD
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Anaphylaxis, major histocompatibility complex, IgE, histamine, IL-4, OVA-lgG1
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