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KMID : 1204320070230030305
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Laboratory Animal Research
2007 Volume.23 No. 3 p.305 ~ p.315
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Proteome Analysis of Rat Testis Using Two-Dimensional Electrophoresis and Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry
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Han Dae-Yong
Lee Hu-Jang
Kim Gon-Sup
Won Chung-Kil
Cho Jae-Hyeon
Park Oh-Sung
Kang Sang-Rim
Kim Eun-Hee
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Abstract
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The testes are essential for male reproduction. This study examined the proteome of the rat testis using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). A total of 244 spots corresponding to 141 different proteins were identified and a two-dimensional electrophoresis protein map was established. The resolution of the map was improved using a 2-DE fractionation kit and the proteins of the rat testis were examined more broadly. This database is expected provide useful basic information for a molecular study of normal and pathologic conditions affecting male reproduction.
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KEYWORD
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Testis, 2-DE, MALDI-TOF MS, proteome
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