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KMID : 1204320070230040405
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Laboratory Animal Research
2007 Volume.23 No. 4 p.405 ~ p.409
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Effects of Coicis on Vaginal Opening and Estrous Cycle in Female Pubertal Onset Assay
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Choi Bong-Ho
Choi Jae-Hong
Kim Yun-Bae
Shin Sun-Hee
Park Dong-Sun
Jeon Jeong-Hee
Jang Min-Jung
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Abstract
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The present study was conducted to investigate the effects of coicis on vaginal opening day, estrous cycle and growth of rats. To examine the parameters mentioned above, we performed a pubertal onset assay using immature Sprague-Dawley female rats. Twenty one-day-old rats were orally administered coicis (125 or 625 ㎎/㎏) or tamoxifen (0.1 ㎎/㎏), an estrogen receptor antagonist, for 30 days. Vaginal opening was observed at 34.43 ㎎/㎏ 1.09 days of age in control rats. The mean vaginal opening day was delayed by about 1 day to 35.30 ± 0.35 days and 35.40 ± 1.13 days by the treatment with 125 ㎎/㎏ and 625 ㎎/㎏ of coicis, respectively. In contrast, tamoxifen significantly advanced the day of vaginal opening to 24 days, indicative of its estrogenic action. Coicis (125 ㎎/㎏) prolonged estrous cycle in 60% of rats, leading to 7.08 ± 3.38 days from 5.78 ± 2.13 days in control animals, although a high dose (625 ㎎/㎏) did not further prolonged it. In contrast, tamoxifen stopped the estrous cycle at diestrus stage. Coicis neither induced clinical signs nor enhanced body weight gain, whereas tamoxifen decreased body weight gain as well as feed and water consumptions. Based on the effects on vaginal opening time and estrous cycle, coicis was confirmed to possess an antiestrogenic activity without adverse effects. Therefore, it is suggested that coicis could be a candidate for the delay of initial menstruation, preventing early reduction in the growth of youngsters in gradually-growing animals or humans.
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KEYWORD
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Coicis, pubertal onset assay, vaginal opening, estrous cycle, growth
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