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KMID : 1204320070230040419
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Laboratory Animal Research
2007 Volume.23 No. 4 p.419 ~ p.425
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Genetic Analysis of KCR as a New Hereditary Cataract Rat
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Park Han-Jin
Cho Kyu-Hyuk
Song Chang-Woo
Kang Min-Sung
Cho Jae-Woo
Cho Jin-Won
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Abstract
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Korea Cataract Rat (KCR) is a hereditary cataract rat in which lens opacitiy appears in the nuclear portion at 2 weeks of age. Phenotypic analysis of KCR lens and linkage analysis were carried out to obtain basic information for cataractogenesis. In histological examination of the lenses, it showed that dysgenesis of anterior suture and formation of numerous vacuoles beside posterior lens capsule. The degeneration in the nuclear region and the swollen vacuoles beside posterior lens capsule were examined in 2 weeks old KCR. For the linkage analysis, male homozygotes of KCRs and female Brown-Norway/SsNHsd rats were mated to produce F₁ hybrids. F₁ progeny were backcrossed to male KCR rats to produce backcross progeny, and were intercrossed to produce F₂ intercross progeny. The segregation ratio between mutants and normal progeny were 1 : 3 and 1 : 1 in F₂ intercross and backcross progeny, respectively (P ＞ 0.05). The results of linkage analysis, the gene responsible for cataractogenesis was strongly linked to D5Rat108, D5Rat164 and D5Rat30 on choromosome (chr) 5. The responsible gene was provisionally named Kcr. In the vinicity of linkage region, we identified that Foxe3 involving in inducing Peters’ anomaly in human and dysgenesis lens in mouse was coded. Foxe3 is considered as a strong candidate gene of Kcr.
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KEYWORD
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KCR, cataract, microsatellite marker, linkage analysis, Foxe3
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