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KMID : 1204320070230040441
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Laboratory Animal Research
2007 Volume.23 No. 4 p.441 ~ p.446
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The Protective Effect of Ginsenosides against High Glucose-Induced Inhibition of Na?/glucose Cotransporter in Primary Cultured Rabbit Proximal Tubule Cells
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Lim Suel-Ki
Yoon Kyung-Chul
Han Ho-Jae
Park Soo-Hyun
Bae Chun-Sik
Yoon Byeong-Cheol
Park Min-Jung
Jung Su-Young
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Abstract
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The development of diabetic nephropathy is associated with the dysfunction of proximal tubule cells. High glucose has also been considered to play an important role in alteration of Na?/glucose cotransporter in proximal tubule cells. Ginsenosides have been used as a remedy for diabetes in Asian countries. Therefore, we examined the preventive effect of ginsenosides against high glucose-induced alteration of Na?/glucose cotransporter and its related signal pathways in the primary cultured proximal tubule cells. In present study, high glucose inhibited Na?/glucose cotransporter (α-MG uptake) in proximal tubule cells. Ginsenosides total saponin (GTS), penoxatriol (PT) and penoxadiol (PD) blocked high glucose-induced inhibition of α-MG uptake in proximal tubule cells. In addition, high glucose stimulated cAMP formation and PKC activity from cytosolic to membrane fraction. GTS, PT and PD prevented high glucose-induced stimulation of cAMP and PKC activity. Moreover, high glucose stimulated TGF-β1 secretion, which was completely blocked by the treatment of GTS, PD and PT in proximal tubule cells. In conclusion, GTS prevented high glucose-induced dysfunction of proximal tubule cells via inhibition of cAMP, PKC, and TGF-β1 signaling cascades.
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KEYWORD
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Ginsenosides, proximal tubule cells, cAMP, protein kinase C, TGF-β1
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