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KMID : 1204320070230040447
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Laboratory Animal Research
2007 Volume.23 No. 4 p.447 ~ p.452
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Epigallocatechin Gallate Protects the Dysfunction of Mesangial Cells in Hyperglycemic Conditions in Vitro
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Han Ho-Jae
Park Soo-Hyun
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Abstract
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Green tea, a beverage commonly consumed in Asian countries, is a significant source of a type of flavonoids called catechins. Epigallocatechin-3-gallate (EGCG) is the most abundant chemical components of these catechins. It has been reported that glomerulosclerosis mediated by the dysfunction of mesangial cells and insulin-like growth factors (IGFs) are associated with the development of diabetic nephropathy. Hyperglycemia is a major factor to develop the onset of diabetic nephropathy. Thus, we examined the effect of EGCG on high glucose-induced dysfunction of primary cultured rat mesangial cells. In the present study, treatment of 25 mM glucose increased IGF-Ⅰ and IGF-Ⅱ secretion in mesangial cells. Supplementation of EGCG (＞1 ㎍/㎖) resulted in the prevention of high glucose-induced increase of IGF-Ⅰ and IGF-Ⅱ secretion in mesangial cells. In addition, treatment of EGCG reduced the formation of lipid peroxide and GSH contents. EGCG also ameliorates high glucose-induced increase of arachidonic acid release and decrease of prostaglandin E₂. In conclusion, EGCG prevented high glucose-induced dysfunction of mesangial cells via inhibition of oxidative stress and arachidonic acid pathways.
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KEYWORD
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Epigallocatechin-3-gallate, mesangial cells, oxidative stress, arachidonic acid, insulin-like growth factor-1
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