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KMID : 1204320070230040453
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Laboratory Animal Research
2007 Volume.23 No. 4 p.453 ~ p.458
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Functional Expression of Riluzole-Sensitive Two-Pore Domain Background Potassium Channels in Dorsal Root Ganglion Neurons of Neonatal Rats
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Park Kyoung-Sun
Kim Chan-Hyung
Kim Yang-Mi
Shin Eun-Young
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Abstract
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Two-pore domain background K? channels are widely believed to play an important role in maintaining and regulating neuronal excitability. We studied whether riluzole-sensitive background K? channels are functionally expressed in dorsal root ganglion (DRG). DRG neurons were dissociated from 1 day rats and electrophysiological recording was performed using a patch-clamp amplifier. Inside-out patches and cellattached membrane patches were voltage clamped at a constant potential (mainly -60 mV) in symmetrical 150 mM K? solutions. The recorded K? channels in DRG neurons was determined to be TRAAK, as judged by its electrophysiological bursting kinetics and activation by membrane stretch, free fatty acids, intracellular alkalosis and riluzole. These data suggest that DRG neurons possess a riluzole sensitive background current which shares several pharmacological and electrophysiological properties with recently cloned two-pore K? channels, particularly TRAAK.
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KEYWORD
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TRAAK, riluzole, Two-pore domain K? channel, dorsal root ganglion neurons, single channel recording
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