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KMID : 1204320070230040471
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Laboratory Animal Research
2007 Volume.23 No. 4 p.471 ~ p.475
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Immunohistochemical Localization of Cell Surface Antigens in Lymphomas Induced by N-Methyl-N-nitrosourea in rasH2 Transgenic Mice
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Lee Gary
Ahn Byeong-Woo
Han Beom-Seok
Choi Mi-Na
Jeong Ja-Young
Jo Hyun-Ye
Kim Mi-Hee
Cho Wan-Seob
Kim Sheen-Hee
Cho Min-Jung
Kim Hyung-Ook
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Abstract
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The strain of rasH2 mice carrying human c-Ha-ras gene had been developed to use for rapid carcinogenicity testing. The mice develops various kinds of tumors including lymphoma when induced by carcinogen N-methyl-N-nitrosourea (MNU). In this study, 30 rasH2-Tg mice were treated with 75 ㎎/㎏ of MNU to induce lymphoma in 26 weeks. A total of 22 mice (13 male, 9 female) developed lymphoma. Immunohistochemistry for localization of cell surface antigens were performed to subclassify the lymphomas. Antibodies against BOB.1 and oct-2 were applied for B cell lymphoma and CD3 for T cell lymphoma. In T cell lymphoma, CD3 were expressed in cytoplasmic membranes of most tumor cells, whereas oct-2 and BOB.1 were localized in some cells in B cell lymphoma. Most of lymphomas cases expressed T cell marker CD3. The reason T cell lymphomas so frequently developed in rasH2 mice by MNU treatment should be further elucidated.
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KEYWORD
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BOB.1, CD3, lymphoma, MNU, oct-2, rasH2 mouse
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