|
KMID : 1204320080240010093
|
|
Laboratory Animal Research
2008 Volume.24 No. 1 p.93 ~ p.97
|
|
|
Anti-allergic Effects of Rose Petal Extract and Ganoderma lucidum Culture on Mast Cell-Mediated Allergy Model
|
|
Kwon Sang-Chul
Kim Chang-Hwan
Kim Yun-Bae
Shin Sun-Hee
Park Dong-Sun
Jeon Jeong-Hee
Jang Min-Jung
Jeong Jae-Hyun
Kim Jwa-Jin
|
|
|
Abstract
|
|
|
|
The effects of rose petal extract (RPE) and Ganoderma lucidum culture (GLC) on the systemic and local anaphylactic reactions induced by compound 48/80 were investigated in mice. To induce systemic anaphylactic shock, male ICR mice were intraperitoneally challenged with a lethal dose (8 ㎎/㎏) of compound 48/80, a mast cell degranulator, 30 min after intraperitoneal administration of RPE or GLC (10, 32 or 100 ㎎/㎏). In comparison with 100% death, within 60 min, of control mice administered with vehicle, the mortalities of mice pretreated with 32 and 100 ㎎/㎏ of RPE were decreased to 20 and 0%, respectively. A dose-dependent decrease in the mortality was also achieved with GLC, resulting in 60, 20 and 0% in mice pretreated with 10, 32 and 100 ㎎/㎏, respectively. In local atopic dermatitis induced by intradermal injection of compound 48/80 (50 ㎍/site), the mice exhibited 169.1±10.5 times of scratching behaviors for 30 min and vascular leakage of Evan’s blue; 118.3±16.9 mm2 in size and 2.61±0.48 μg in amount. The scratching behaviors induced by compound 48/80 was significantly attenuated by 6-day oral pretreatment with RPE (300 ㎎/㎏) to 86.9±16.4 times. In addition, Evan’s blue leakage was also greatly reduced by RPE. In contrast, GLC did not decrease both the number of scratching behaviors and dye leakage, implying that different factors other than histamine are also involved in the atopic dermatitis induced by compound 48/80. Taken together, it is suggested that RPE contains ingredients inhibiting degranulation or action of inflammatory mediators from mast cells, and that could be a promising candidate for the prevention of systemic or local allergic reactions including atopic dermatitis.
|
|
|
KEYWORD
|
|
|
Rose petal extract(RPE), Ganoderma lucidum culture(GLC), compound 48/80, anaphylaxis, atopic dermatitis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|