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KMID : 1204320080240020249
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Laboratory Animal Research
2008 Volume.24 No. 2 p.249 ~ p.255
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Expression Profile of Glutathione Peroxidase Genes in the Male Reproductive System of Mice Exposed to Ginseng Extracts
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Kim Mi-Ra
Nam Sang-Yoon
Lee Beom-Jun
Kim Yun-Bae
Baek In-Jeoung
Yon Jung-Min
Lee Se-Ra
Yun Young-Won
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Abstract
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Ginseng is one of the most common herbal medicines in the Orient which has been used for the treatment of disease due to its wide spectrum of medicinal effects, such as tonic, immunomodulatory, antioxidant, and anti-aging activities. However, there are only a few reports on the effects of Korean ginseng on the reproductive organs in animals and humans. To understand the antioxidative effect of Korean ginseng on the male reproductive system, we analyzed the mRNA expression patterns of glutathione peroxidase (GPx) family [cytosolic (GPx1), gastrointestinal (GPx2), and phospholipid hydroperoxide (GPx4) types] in the testes, epididymes, prostates, and seminal vesicles of mice exposed to Korean ginseng. Korean ginseng extracts (20, 200, and 2,000 mg/kg body weight) were treated to 7-weekold male mice for 9 weeks daily per oral. Total RNAs were extracted from testis, epididymis, prostates, and seminal vesicles, and then RT-PCR was performed with the specific primers of each GPx genes. In testis, all type of GPx mRNA expression was similar to that of control group. However, the gene expression of GPx2 was decreased in the epididymis exposed to 2,000 ㎎/㎏ of Korean ginseng, but it was no significant difference. The gene expression of GPx1 in prostates was tend to decrease slightly compare to control group, but those of GPx2 and GPx4 were weakly increased by Korean ginseng treatments. On the contrary, the gene expressions of GPx2 and GPx4 in seminal vesicles were decreased slightly compare to control group. These findings suggest that Korean ginseng may influence the antioxidant system of male reproductive organs in a differential manner.
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KEYWORD
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Korean ginseng, glutathione peroxidase (GPx), male reproductive organs
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