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KMID : 1204320080240030355
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Laboratory Animal Research
2008 Volume.24 No. 3 p.355 ~ p.360
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Protective Effect of Anthocyanins against the Hepatotoxicity of Carbon Tetrachloride in Rats
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Shin Sun-Hee
Park Dong-Sun
Jang Min-Jung
Jeon Jeong-Hee
O Nam-Gil
Song Ju-Gyeong
Lee Jin-Soo
Kim Byung-Yul
Joo Seong-Soo
Hwang Seock-Yeon
Kim Yun-Bae
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Abstract
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Reddish purple-colored fruits and vegetables contain high level of anthocyanins, which are well known to exert antioxidant, cardioprotective and hepatoprotective actions. In the present study, the effect of purified anthocyanin on the liver injury and dysfunction induced by carbon tetrachloride (CCl₄) was investigated. Male Sprague-Dawley rats were injected intraperitoneally with CCl₄ at a dose of 1.1 mL/㎏ to induce hepatic damage. The rats were orally administered with anthocyanin (50, 160 or 500 ㎎/㎏) 4 hr and 30 min prior to as well as 4 hr and 20 hr after CCl₄ challenge. Four hr following final anthocyanin treatment (24 hr after CCl₄ injection), time-course of blood concentration of intravenously-injected bromosulphalein were examined. In addition, blood parameters of hepatic injury in accordance with histopathological findings were analyzed. The biliary excretion time and clearance of bromosulphalein were greatly delayed by CCl₄ injection, which were recovered by anthocyanin (160-500 ㎎/㎏). CCl₄ drastically increased the blood markers of hepatotoxicity, and caused centrilobular congestion, hepatocytic degeneration, lipid droplets and inflammatory cell infiltration, leading to disintegration of hepatic cords The increased biochemical parameters and hepatic lesions induced by CCl₄ were significantly attenuated by anthocyanin treatment. The results suggest that anthocyanin might prevent liver dysfunction induced by CCl₄, and that anthocyanin could be a potential candidate for the prevention of hepatic disorders.
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KEYWORD
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Carbon tetrachloride (CCl4), liver dysfunction, hepatic disorders, anthocyanin
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