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KMID : 1204320080240030361
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Laboratory Animal Research
2008 Volume.24 No. 3 p.361 ~ p.369
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Effect of Repeated Administration of Silk Peptide on the Immune System of Rats
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Ryu Jae-Myun
Kim Tae-Myoung
Seo Im-Kwon
Yeon Seong-Ho
Lim Woo-Taek
Lee Jeong-Yong
Hwang Seock-Yeon
O Nam-Gil
Song Ju-Gyeong
Lee Jin-Soo
Kim Yun-Bae
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Abstract
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In order to elucidate the possibility of influence on the immune system, silk peptide was administered to rats for 4 weeks. Male and female Sprague-Dawley rats were orally treated with low (500 ㎎/㎏) or high (2,000 ㎎/㎏) doses of silk peptide, and complete blood counts, organ weights, histopathology and cellularity of immune systems were examined. Silk peptide did not cause clinical signs, although the body weight gain at the high dose was slightly reduced. In hematological analysis, the number of white blood cells (WBC) and differential counts, the number of red blood cells (RBC) and RBC indices, and the number of platelets and mean platelet volume (MPV) were not affected by the administration of silk peptide. Especially, there were no differences in the number and rate (%) of immune cells such as lymphocytes as well as monocytes and granulocytes between vehicle and silk peptide-treated groups. Silk peptide neither affected the thymus and spleen weights, in addition to the cellularity in thymus, nor induced gross and microscopic lesions of thymus, spleen and lymph node. Taken together, 4-week administration of silk peptide up to 2,000 ㎎/㎏, the upper-limit dose in repeated-dose toxicity study, did not exhibit adverse effects on the immune systems, leading to no observed adverse effect level (NOAEL) higher than 2,000 ㎎/㎏. Without influence on the production, maturation and death process of immune cells at 20-fold dose of clinical dose (100 ㎎/㎏), silk peptide was evaluated to be a practically non-toxic material.
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KEYWORD
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Silk peptide, repeated-dose toxicity, immune system
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