|
KMID : 1204320080240030399
|
|
Laboratory Animal Research
2008 Volume.24 No. 3 p.399 ~ p.404
|
|
|
Inhibition of Phthalic Anhydride-Induced Skin Inflammation in IL-4 Knock-out Mice
|
|
Bae Chang-Jun
Kim Mi-Ran
Kim Chul-Kyu
Kim Byung-Guk
Shim Sun-Bo
Lee Su-Hae
Sin Ji-Soon
Woo Jong-Min
Choe Nong-Hoon
Ryoo Zae-Young
Lee Chang-Kyu
Chae Kab-Ryong
|
|
|
Abstract
|
|
|
|
Atopic dermatitis and asthma are common chronic inflammatory diseases. A pivotal role of IL-4 in airway inflammation has been elucidated by studying mice in which IL-4 was genetically disrupted. However, there have been no trials to examine the effect of IL-4 dysfunction on skin inflammation. Thus, the aim of this study was to investigate the role of IL-4 in skin inflammation using IL-4-/- mice. To induce skin inflammation, a 15% phthalic anhydride solution was repeatedly applied to the dorsal surfaces of the ears of both IL-4-/- and BALB/c mice for 5 weeks. After treatment, skin inflammation-related factors were detected in the ear tissue, lymph nodes, spleens, and sera of the mice. Ear thicknesses and weights of the auricular lymph nodes, indicating the degree of skin inflammation, were significantly smaller in IL-4-/- mice than in BALB/c mice. Immunoglobulin E concentrations in sera were also significantly lower in IL-4-/- mice. In cytokine secretion profiles, expression levels of inflammation-inducing cytokines and chemokines, such as IL-6, IL-10, IL-13, KC, MCP-1 and VEGF were lower in IL-4-/- mice than in BALB/c. These findings suggest that knock-out of IL-4 relieves allergen-induced skin inflammation. Thus, the modulation of IL-4 expression may provide important insights into therapeutic targets of chronic skin inflammatory diseases, such as atopic dermatitis.
|
|
|
KEYWORD
|
|
|
IL-4, allergic disease, cytokine, chemical allergen
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|