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KMID : 1204320080240030421
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Laboratory Animal Research
2008 Volume.24 No. 3 p.421 ~ p.428
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Phytic Acid Increases the Formation of Colonic Aberrant Crypt Foci in Male F344 Rats with Iron-Deficient Status
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Lee Yea-Eun
Hue Jin-Joo
Lee Ki-Nam
Nam Sang-Yoon
Ahn Byeong-Woo
Yun Young-Won
Jeong Jae-Hwang
Lee Beom-Jun
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Abstract
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Iron is essential for life and has numerous biochemical roles. Phytic acid (PA) has been recognized as a natural antioxidant by its chelating properties and reducing the catalytic activities of many divalent transition metals. We investigated the effect of PA on the formation of aberrant crypt foci (ACF) induced by azoxymethane (AOM) in dietary iron-deficient F344 rats. After acclimation for one week, six-week old F344 male rats were fed with the AIN-93G purified diet (normal diet) or an iron-deficient diet (3 ppm Fe, only 10% vs control diet) for 8 weeks. PA (0.5% or 2% PA in water) was given for drink throughout the experimental period. Animals were treated subcutaneously with AOM (15 ㎎/㎏) twice (1st and 2nd week of the experiment) to induce colonic ACF. After sacrifice, the colonic mucosa were examined for the total numbers of aberrant crypt (AC) and ACF after staining with methylene blue. The blood and serum were analyzed with a blood cell differential counter and an automatic serum analyzer. Iron-deficient diet induced an anemic status of rats as indicated by significantly low values in RBC count, hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin, compared with the AOMtreated control (P＜0.01). However, treatments of PA increased these values. Treatment with 2% PA significantly increased the numbers of both ACF/colon and AC/colon in iron deficient rats, compared with the respective control (P＜0.05). The number of large ACF (≥4 AC/ACF), which had been suggested to possess a greater tumorogenic potential than small ACF (≤3 AC/ACF), was significantly enhanced by treatment with 2% PA (P＜0.05). These results suggest that PA may promote colon carcinogenesis in irondeficient status in rats.
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KEYWORD
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Colon cancer, phytic acid, azoxymethane, aberrant crypt foci, iron-deficient rat
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