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KMID : 1204320080240040565
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Laboratory Animal Research
2008 Volume.24 No. 4 p.565 ~ p.573
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Four-Week Repeated-Dose Toxicity of Silk Amino Acids in Rats
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Kim Tae-Myoung
Ryu Jae-Myun
Seo Im-Kwon
Yeon Seong-Ho
Lim Woo-Taek
Lee Jeong-Yong
Hwang Seock-Yeon
Kim Yun-Bae
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Abstract
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This study was performed to confirm the safety of the silk amino acids (SAA). Male and female Sprague-Dawley rats were orally administered with SAA at dose levels of 500, 1,000 or 2,000 ㎎/㎏ for 4 weeks. There was no significant difference in the body weight gain between vehicle control and SAA-treated rats. Also, significant changes in daily feed and water intakes were not observed. In hematological analysis, SAA decreased the rates of monocytes and eosinophils, while increasing that of lymphocytes, suggestive of reduced inflammatory response and immune potentiation. In particular, SAA significantly increased the numbers of red blood cells, showing microcytic erythropoiesis, and platelets, implying an enhanced hematopoiesis. In blood biochemistry, alanine transaminase, aspartate transaminase and lactate dehydrogenase were significantly reduced by SAA treatment, in contrast to the increases in glucose and albumin. Blood urea nitrogen and creatine phosphokinase also significantly decreased following SAA treatment. In spite of the decreases in the markers of liver, kidneys, skeletal and heart muscles, no remarkable lesions were observed in these organs. Such effects of SAA on the blood markers might be due to the stabilization or protective activities on the active organs, which may lead to enhanced resistance to hyperactivity, hypermotility or excitatory stress. Since no observed adverse-effect level of SAA was found to be higher than the upper-limit dose (2,000 ㎎/㎏), a 20-fold higher level of a clinicallyrecommended dose (100 ㎎/㎏), it is suggested that SAA possess a long-term safety, and that it could be a candidate for the improvement of hematopoiesis and stamina.
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KEYWORD
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Silk amino acids (SAA), repeated-dose toxicity
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Listed journal information
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