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KMID : 1204320080240040601
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Laboratory Animal Research
2008 Volume.24 No. 4 p.601 ~ p.607
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Effect of Phytic Acid on the Formation of Colonic Aberrant Crypt Foci induced by azoxymethane in Fe-deficient ICR Mice
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Hue Jin-Joo
Han Ji-Yeon
Lee Ki-Nam
Miga Myak
Kim Jong-Soo
Nam Sang-Yoon
Ahn Byeong-Woo
Yun Young-Won
Jeong Jae-Hwang
Lee Beom-Jun
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Abstract
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Phytic acid (PA) has chelating and reducing properties on many divalent transition metals. Its properties may be associated with a role in anticancer activity. In the present study, effects of PA on colon carcinogenesis were investigated in Fe-deficient ICR male mice. Five-week old mice were acclimated for one week and assigned to five experimental groups including normal control, azoxymethane (AOM) only, AOM+low Fe, AOM+low Fe+0.5% PA, and AOM+low Fe+2% PA groups. Animals fed on iron-normal diet (35 ppm Fe) or iron-deficient diet (3 ppm Fe) and PA (0.5% or 2% PA in water) for 8 weeks. Animals received three (0-2nd weeks after starting experiment) injections of AOM (10 ㎎/㎏ bw) to induce colonic aberrant crypt foci (ACF). The total numbers of ACF and aberrant crypt (AC) were measured in the colonic mucosa stained with methylene blue. The blood and serum were analyzed with a blood cell differential counter and an automatic serum analyzer. Low iron diet induced a significant low in hemoglobin and mean corpuscular volume and hemoglobin values, compared with normal diet group (P＜0.05). AOM treatment induced total numbers of 81.7 ACF and 102.0 AC per colon in normal iron-diet (AOM alone) group. Low dietary iron (AOM LFe) decreased the numbers of ACF (64.9) and AC (79.8) per colon by 20.6 and 21.8%, respectively, compared with AOM alone group. Treatment of 0.5% PA significantly decreased the numbers of ACF (50.3) and AC (60.5) per colon by 22.5 and 24.2%, respectively, compared with AOM LFe group (P＜0.05). PA at 2% also reduced the formation of ACF or AC by 13.3 and 18.3%, respectively, compared with AOM LFe group (P＜0.05). No detrimental effects by PA treatment were observed in blood and serum chemistry, body weight, and relative organ weight of mice during the experimental period. These findings suggest that PA may prevent colon carcinogenesis in mice even with iron-deficient status.
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KEYWORD
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Colon cancer, phytic acid, azoxymethane, aberrant crypt foci, iron-deficient mice
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