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KMID : 1204320080240040659
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Laboratory Animal Research
2008 Volume.24 No. 4 p.659 ~ p.667
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The Modulation of Cell Wall-Associated Triton X-100 Soluble Protein (TSP) Antigens of Mycobacterium tuberculosis on Murine Dendritic Cells
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Yoo Young-Choon
Bae Young-Joo
Paik Tae-Hyun
Lee Jung-Lim
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Abstract
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Mycobacterium tuberculosis or its secreted protein antigens are known as a potent inducer of maturation process of dendritic cells, during which the expression of cell surface molecules and the capability of antigen presentation are increased, resulting in potent interaction with T cells to initiate the acquired immune response against tuberculosis. In this study, the cell wall-associated Triton X-100 soluble protein (TSP) antigens, TSP- BCG, TSP-H37Ra, TSP- H37Rv and TSP-K, were tested on murine bone marrowderived dendritic cells to determine the capability of their immunologic modulation. Only TSP-H37Rv stimulated murine dendritic cells showed moderate production of IL-6 and MIP-1α compared to those of LPS. TSP-H37Rv antigen did not induce the production of IL-12, TNF-α or IL-10. Other TSP antigenstimulated murine dendritic cells showed minimal or no induction of cytokines (IL-6, IL-12, TNF-α or IL-10) or chemokines (MIP-1α or RANTES). The murine dendritic cells showed the production of NO as well as the expression of iNOS mRNA after LPS stimulation, however, none of TSP antigen-stimulated murine dendritic cells induced either NO or iNOS mRNA expression. The allostimulation of T cells by murine dendritic cells, which were stimulated with each TSP antigens, showed insignificant increase in T cell proliferation activity in mixed lymphocyte reaction. These results suggested that cell wall-associated TSP antigen isolated from Mycobacterium tuberculosis H37Rv strain acts as a more potent stimulator on murine dendritic cells than any other TSP antigens, even the stimulation from TSP-H37Rv was not enough to activate the maturation of process of murine dendritic cells.
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KEYWORD
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Mycobacterium tuberculosis, TSP antigens, murine dendritic cells, IL-6
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