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KMID : 1204320090250010035
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Laboratory Animal Research
2009 Volume.25 No. 1 p.35 ~ p.40
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Protective Effect of an Herbal Preparation (HemoHIM) on 5-Fluorouracil-Induced Intestinal Injury in Mice
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Lee Hae-June
Kim Joong-Sun
Moon Chang-Jong
Kim Jong-Choon
Bae Chun-Sik
Kang Sung-Soo
Jung U-Hee
Park Hae-Ran
Jo Sung-Kee
Kim Seong-Ho
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Abstract
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The morphological changes of small intestine and protective properties of an herbal preparation (HemoHIM) in intestinal damages were examined by evaluating its effects on jejunal crypt survival, apoptosis and other histological assessment in 5-fluorouracil (5-FU)-treated mice. The female ICR mice were treated with 5-FU for the examination of jejunal crypt survival and any morphological changes (200 ㎎/㎏ of body weight, i.p.), and for the detection of apoptosis (40 ㎎/㎏ of body weight, i.p.). HemoHIM was administered intraperitoneally at a dosage of 50 ㎎/㎏ of body weight at 36 and 12 hours preirradiation and 30 minutes post-irradiation, or orally at a dosage of 250 ㎎/㎏ of body weight/day for 7 or 11 days before autopsy. Compared to normal controls, 5-FU increased the incidence of apoptosis by 20-fold. 5-FU decreased villus height in the jejunum (44.6%) with crypt depths increased by 18.6% in this gut region. In comparison with normal controls, the values of basal lamina length of 10 enterocytes in the jejunum were significantly higher in 5-FU treated mice (55.5%). These effects were less profound in HemoHIM treated mice in which apoptosis was decreased 11.4% (p.o.) and 27.4% (i.p.), with villus height increased by 37% (p.o.) and 28% (i.p.), crypt depth by 13.4% (p.o.) and 19.0% (i.p.) and accompanied by decreases in basal lamina length of 23.5% (p.o.) and 31.9% (i.p.), compared to 5-FU control group. These results suggested that HemoHIM may be therapeutically useful to reduce intestinal injury following 5-FU treatment.
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KEYWORD
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Apoptosis, HemoHIM, intestine damage, 5-fluorouracil
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