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KMID : 1204320090250010051
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Laboratory Animal Research
2009 Volume.25 No. 1 p.51 ~ p.57
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Effect of Phytic Acid on the Formation of Colonic Aberrant Crypt Foci induced by Azoxymethane in Fe-overloaded Mice
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Hue Jin-Joo
Kang Bong-Su
Kim Jong-Soo
Nam Sang-Yoon
Ahn Byeong-Woo
Yun Young-Won
Lee Beom-Jun
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Abstract
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There are accumulating evidences that high levels of dietary iron may play a role in colon carcinogenesis. Phytic acid (PA), a natural antioxidant, has chelating and reducing properties on many divalent transition metals. The effects of PA on colon carcinogenesis were investigated in Fe-overloaded ICR male mice. Fiveweek old mice were acclimated for one week and fed on iron-normal diet (35 ppm Fe) or iron-overloaded diet (350 ppm Fe) for 8 weeks. Animals received three (0-2nd weeks after starting experiment) injections of azoxymethane (AOM; 10 mg/kg b.w.) to induce colonic aberrant crypt foci (ACF). There were five experimental groups including normal control, AOM only, AOM+high Fe, AOM+high Fe+0.5% PA, and AOM+high Fe+2% PA groups. PA was given by drinking water for 8 weeks. The total numbers of ACF and aberrant crypt (AC) were measured in the colonic mucosa after staining with methylene blue. The effects of PA during colon carcinogenesis were analyzed by measuring the total number of aberrant crypt (AC) and ACF in the colonic mucosa, and the hematological and serum biochemical values. High-iron diet induced a significant high in iron content in the livers of mice (P＜0.05). Iron-overload diet did not change hematological and serum chemical values compared with normal-diet in ICR mice. There were no significant differences in relative organ weights of mice among experimental groups. High-iron diet increased ACF (82.9±11.5) and AC (96.0±12.3) formation, compared with normal-diet (72.4±15.4 ACF/colon and 90.3±20.1 AC/colon). PA reduced the number of ACF and AC per colon in a dose-dependent manner. 0.5% and 2% PA significantly decreased the numbers of ACF per colon by about 47.5% (43.5±7.2) and 54.3% (37.9±5.5), respectively (P＜0.05). The numbers of AC were also significantly reduced by the treatments of PA (P＜0.05). These results suggest that phytic acid can be a chemopreventive agent for colon cancer in animals and humans even with iron-overloaded status.
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KEYWORD
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Aberrant crypt foci, azoxymethane, colon cancer, phytic acid, iron-overloaded mice
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