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KMID : 1204320090250010075
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Laboratory Animal Research
2009 Volume.25 No. 1 p.75 ~ p.78
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Simple Genotyping Method Using Ampdirect® Plus and FTA® Technologies - Application to the Identification of Transgenic Animals and Their Routine Genetic Monitoring
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Nakanishi Satoshi
Kuramoto Takashi
Serikawa Tadao
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Abstract
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A novel genotyping method, the Amp-FTA method, has been developed by combining the advantages of the Ampdirect® Plus and FTA® technologies. This method allowed the direct PCR amplification of DNA from unpurified blood immobilized on the FTA® card. PCR templates were prepared only by punching out the discs from the FTA® card, which could lead to a foolproof way of genotyping. The PCR products obtained could be subjected to downstream analyses such as restriction enzyme treatment and direct sequencing. The Amp-FTA method was applicable to buccal swabs collected by a simple technique minimizing distress to animals. Therefore, the Amp-FTA method is ideal for the genotyping and genetic monitoring of laboratory animals.
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KEYWORD
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Genotyping, transgenic, monitoring, Ampdirect®, FTA® technology
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