|
KMID : 1204320090250020107
|
|
Laboratory Animal Research
2009 Volume.25 No. 2 p.107 ~ p.111
|
|
|
Estrogen Treatment Attenuates Apoptotic Cell Death Following Spinal Cord Injury in Rats
|
|
Cho Eun-Hae
Sung Jin-Hee
Kim Myeong-Ok
Min Won-Gi
Won Chung-Kil
Cho Jae-Hyun
Lee Hyo-Jong
Koh Phil-Ok
|
|
|
Abstract
|
|
|
|
Estrogen exerts a neuroprotective effect by reducing apoptotic cell death in both brain injury and spinal cord injury. This study investigated whether estrogen contributes to the cleavage of poly (ADP-ribose) polymerase (PARP), an indicator of apoptotic cell death, during spinal cord injury. Adult female rats were ovariectomized and treated with oil or estrogen prior to spinal cord injury. Spinal cord tissues were collected at 24 hr after injury. Estrogen treatment showed a significant improvement of spinal cord tissue morphology and markedly reduced numbers of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Using Western blot analysis, we found that the level of cleaved PARP was increased in oil-treated animals, whereas estrogen prevented the injury-induced increase in PARP cleavage. The results of this study provide further evidence that estrogen inhibits apoptotic cell death through the down-regulation of cleaved PARP in spinal cord injury.
|
|
|
KEYWORD
|
|
|
Estrogen, neuroprotection, spinal cord injury
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|