|
KMID : 1204320090250020151
|
|
Laboratory Animal Research
2009 Volume.25 No. 2 p.151 ~ p.157
|
|
|
Extract of Diospyros kaki Stem Reduces Neuronal Damage in the Gerbil Hippocampus Induced by Transient Cerebral Ischemia
|
|
Yoo Ki-Yeon
Park Ok-Kyu
Lee Choong-Hyun
Choi Jung-Hoon
Won Moo-Ho
Pai Tong-Kun
Hwang In-Koo
Kim Jong-Dai
|
|
|
Abstract
|
|
|
|
Diospyros kaki (Persimmon) fruits and leaves have been used as a traditional medicine and include a number of therapeutic components. The present study investigated neuroprotective effect of the extract of Diospyros kaki stem (EDKS) against ischemic damage in the gerbil hippocampal CA1 region after 5 min of transient cerebral ischemia. To elucidate the neuroprotective effect of EDKS on ischemic damage, 50 mg/kg EDKS was administered orally to gerbils for 7 days before the ischemic surgery, and we used cresyl violet and Fluoro-Jade C (F-J C) staining. In the EDKS-treated group, many survived pyramidal neurons in the CA1 region was stained with cresyl violet and a few degenerative pyramidal neurons was stained with F-J C. The survival rate of the CA1 pyramidal neurons was about 62% compared to that in the vehicletreated group. In the EDKS-treated group, isolectin B4 (a marker for activated microglia) immunoreactivity was significantly lower than that in the vehicle-treated group. These results suggest that repeated supplement of EKDS can protect neurons from ischemic damage.
|
|
|
KEYWORD
|
|
|
Diospyros kaki stem, delayed neuronal death, neuroprotection, hippocampal CA1 region, gerbil
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|