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KMID : 1204320090250030213
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Laboratory Animal Research
2009 Volume.25 No. 3 p.213 ~ p.217
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Melatonin Prevents the Injury-Induced Decreases of Peroxiredoxin-2 and Thioredoxin in Brain Tissue and HT22 Cells
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Koh Phil-Ok
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Abstract
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Peroxiredoxin-2 and thioredoxin exert neuroprotective effects against oxidative stress. Melatonin acts as an antioxidant agent and protects neuronal cells against ischemic brain damage. This study investigated whether melatonin regulates peroxiredoxin-2 and thioredoxin levels in middle cerebral artery occlusion (MCAO)-induced injury and glutamate exposure-induced neuronal cell death. Adult male rats were treated with vehicle or melatonin prior to MCAO. Brains were collected at 24 hr after MCAO and the cerebral cortices were isolated. Proteomics and Western blot analyses demonstrated that ischemic injury induces decreases in peroxiredoxin-2 and thioredoxin proteins. Melatonin pretreatment prevented the injury-induced decreases in peroxiredoxin-2 and thioredoxin. Glutamate exposure induced decreases in peroxiredoxin-2 and thioredoxin in hippocampal-derived cell lines, and melatonin pretreatment prevented the glutamate toxicity-induced decreases in peroxiredoxin-2 and thioredoxin. These results suggest that melatonin mediates neuroprotective effects during neuronal cell damage by preventing injury-induced decreases in peroxiredoxin-2 and thioredoxin.
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KEYWORD
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Melatonin, neuroprotection, peroxiredoxin-2, thioredoxin
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