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KMID : 1204320090250030233
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Laboratory Animal Research
2009 Volume.25 No. 3 p.233 ~ p.236
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High Titer Production of Equine Herpes Virus-1 Using MDBK Cell Culture System
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Jeon Kyong-Leek
Cho Soon-Beom
Lim Yoon-Kyu
Ju Hong-Gu
Jee Young-Heun
Yun Young-Min
Lee Joo-Myoung
Hwang Kyu-Kye
Kim Ok-Jin
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Abstract
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Equine herpesvirus-1 (EHV) is one of the major causes of economic loss in the equine industry. High titer production of the viral antigen is essential for vaccine development and diagnostic applications. In order to produce high titer EHV-1, we investigated various culture conditions such as amount of virus inoculation, cell population, timing of virus harvesting, and the fetal bovine serum (FBS) content of culture media using Madin-Darby bovine kidney (MDBK) cell. We obtained highest titer of EHV when 80% mono-layered MDBK cells were infected with 1 multiplicity of infection (MOI) and the culture supernatant were harvested 36 hours post-infection. The virus titer was higher for media containing 2% FBS than for serum-free media. Conclusively, we could produce EHV up to 1.4×10¹¹ plaque forming unit (PFU)/mL in culture supernatant.
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KEYWORD
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Equine herpesvirus, MDBK cell, high titer production, multiplicity of infection, harvest timing
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