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KMID : 1204320090250030257
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Laboratory Animal Research
2009 Volume.25 No. 3 p.257 ~ p.261
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The Functional Possibility of Ubiquitin Proteasome System in Glial Cells for New Treatment Strategy
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Kim Sok-Ho
Shim Se-Hwan
Shin Gee-Wook
Kwon Jung-Kee
Lee Ki-Chang
Kim Min-Su
Kim Nam-Su
Kwon Young-Bae
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Abstract
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Neurodegenerative diseases are characterized by the accumulation of misfolded proteins that adversely affect neuronal connectivity and plasticity, and trigger cell death signaling pathways. The ubiquitin proteasome system (UPS) is the main intracellular proteolytic system, responsible for the selective removal of damaged and unfolded proteins. Recently many evidences demonstrated that the UPS function degeneration can enhance the processing of neurodegenerative diseases. In the present study, we have showed the functional possibility of the UPS in vitro at the cellular leveling glial cells. In the highly purified glial cultures, astrocytes and microglia, most of these cells were positive for GFAP and CD11b, a specific marker for astrocytes and microglia, respectively. Ubiquitin and ubiquitin C-terminal hydrolase L1 (UCH-L1) is one of the UPS components. Immunocytochemistry analysis showed the expression of ubiquitin and UCH-L1 in the GFAP and CD11b-immunoreactive cultured cells, respectively. On the basis of this evidence, we first suggest that the UPS might play a role in glial cells, astrocytes and microglia. Although little is known about UPS functions in glial cells, our reports have indicated that UPSmediated neurodegenerative diseases can be investigated for glial proteolytic dysfunction.
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KEYWORD
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Glial cell, astrocytes, microglia, ubiquitin, UCH-L1, UPS
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