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KMID : 1204320100260010015
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Laboratory Animal Research
2010 Volume.26 No. 1 p.15 ~ p.20
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Investigations of Samwhang-sasimtang Extracts on Biological Activities In Vitro and Vivo
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Lee In-Chul
Kim Bae-Hwan
Kim Mee-Kyung
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Abstract
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In this study, we investigated the in vitro antioxidative effects, antimicrobial activities and single oral dose toxicity of the extracts from Samwhang-sasimtang to evaluate its use as a functional ingredient in cosmetics. In the antioxidative effect, the ethanol extract from Samwhang-sasimtang (SSE) had higher antioxidant values of 91.9% at 1,000 ㎍/mL than that of water extract from Samwhang-sasimtang (SSW, 77.0%) when evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. 2,2’-azinobis( 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization of SSE was 82.2%, higher than that of the SSW (55.0%) and the antioxidant protection factors (APF) of SSW and SSE were 1.64 and 1.62 at 1,000 ㎍/mL in concentration, respectively. This study was also undertaken to test the in vitro antimicrobial activity with the extracts of Samwhang-sasimtang. In general, the SSE showed the significant antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis. In single oral dose toxicity study, there were no differences in vivo were observed between control and treated groups in clinical signs, body weight gains, and gross finding. The results indicated that SSE did not show any toxic effects at 10 mL/㎏ in mice, and the LD?? of SSE was found to be higher than 10 mL/㎏ in this experiment. In conclusion, the extracts from Samwhang-sasimtang may act as a natural subsistence for functional cosmetics.
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KEYWORD
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Samwhang-sasimtang, antioxidative activity, antimicrobial activity, single oral dose toxicity
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