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KMID : 1204320100260010109
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Laboratory Animal Research
2010 Volume.26 No. 1 p.109 ~ p.115
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Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray
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Yu Saet-Byeol
Kim Sung-Chan
Kim Jae-Bong
Hwang Yoo-Seok
Kim Jong-Il
Lee Ok-Joo
Park Young-Ju
Lee Sung-Young
Lee Seung-Hwan
Yoon Jae-Ho
Lee Jae-Yong
Park Jae-Bong
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Abstract
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Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
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KEYWORD
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Xenopus, BMP inhibition, neurogenesis, Affymetrix gene chips
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